FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314844

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314844
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1292961
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGCG1106.7770184E-445.338142145
CTACTCG5350.033.0725252
TACTCGG7800.027.2212313
CGTCGAG1050.00867012126.9619852
CGAGTAG4750.025.3300723
CCGATAC2051.555289E-524.1671432
GACTTGT10800.023.5917343
ACTCGGG9650.023.4694984
TGATCAA11750.022.8899
GCTACTC15250.022.7408831
CTTGTGA12350.021.2039075
GAGACTT12700.021.1768321
CTCGGGA11200.020.8534095
AAAGTGC22350.020.5833934
ACTTGTG13100.020.5302124
TCGGGAG11350.019.9542436
ATTCTCG3200.005217758519.481232145
AAGTGCT23800.019.3293615
CCGAGTA6650.019.1571982
AGACTTG14050.019.142052
Produced by FastQC (version 0.11.5)