FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314847

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314847
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1102783
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTTA455.734572E-498.4784145
CCCGTAA900.004718284630.5385041
GGTAACG950.006145612328.9312131
CTACTCG5850.028.1893862
TACTCGG7000.026.5030563
TTTGGCG1901.0854059E-525.3148124
TACATCG1953.7584722E-421.142045
CCTATAT2605.103322E-621.142041
GCTACTC13450.019.9238191
GGTCTAT2157.2581746E-419.175341
AAAGTGC19200.018.60944
GTGCGGA4100.00819519618.014343145
TAGCCTA2400.001518728317.1779087
GATAGTA2802.150194E-417.1779081
TCGGGAG9300.016.9931986
AAGTGCT20350.016.8824655
CCAAAGT20350.016.5448172
ACTCGGG9650.016.3768654
CTACTTG8500.016.1674422
CTCGGGA9950.015.8830895
Produced by FastQC (version 0.11.5)