FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314850

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314850
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences799491
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG3450.034.933392
TATATCG850.003059782333.3619967
GTCGTAT900.004048221331.5085541
TACTCGG4700.027.1509843
CGGAGGT4900.024.595967
TCGTATT1450.001397185424.446292
AAAGTGC14300.023.7966674
GCTACTC10000.023.3950981
AGGCGGA8800.022.5572574
ACTCGGG6250.021.551854
CCCAAAG19850.021.071841
AAGTGCT16250.020.9410695
CTAACTA2054.263891E-420.7495332
CGAGTAG3551.2843157E-719.9702073
CCAAAGT18550.019.8733162
GCTACTT7300.019.423081
CCATAGT4052.431807E-819.2552261
TTGGGAG17750.019.1714026
CTACTTG7400.019.1606062
GGAGGCT25250.019.092319
Produced by FastQC (version 0.11.5)