FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314853

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314853
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences840419
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATGCG950.00527005629.8547535
CCCGTAA1000.00677102228.3620171
TTGGGAG19000.026.122916
CGAGTAG3701.2732926E-1124.912583
CTACTCG3801.8189894E-1124.2569872
TACTCGG5350.023.85593
GCACTTT13650.021.8169351
GCTACTT8800.021.7549531
AAAGTGC16100.021.5797964
ACTTTGG15400.021.1794283
CCGAGTA4701.4551915E-1121.1206512
CTACTTG8150.020.8800132
GGAGGCT26900.020.8234129
ATTCGGG2054.2597586E-420.7526957
GGGAGGC35600.020.3154898
TCGGGAG8400.020.2585856
TAGTATG2105.0115894E-420.2585857
TTACTCG1750.00416128520.2585832
GCTAATA3159.0122376E-720.2585831
TGGGAGG33850.020.1089637
Produced by FastQC (version 0.11.5)