FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314854

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314854
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1221328
Sequences flagged as poor quality0
Sequence length36-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCCG1400.001515593738.495872145
CGTCGAG2151.8189894E-1135.9432331
ACCGGTC850.003199814633.0600859
TTCGGCG850.003199814633.0600855
CGAGTAG4700.026.9052813
GTCGAGA3101.2387318E-924.9283712
CTACTCG5800.023.0138112
GACTTGT11000.022.9917874
CCGTTTT3100.002769633721.73154145
AGTAGCT15300.020.6625545
GCTAACT3108.452662E-720.395941
ATAACGT2156.2521826E-419.60544
TGATCAA11900.019.4818369
CCGAGTA7600.019.4119242
GAGACTT12550.019.0325981
GAGTAGC11150.018.9020674
CGAGACT12500.018.546711
GCTACTC14650.018.222541
AAAGTGC22000.018.2018324
ACTTGTG14300.018.1772674
Produced by FastQC (version 0.11.5)