FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314856

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314856
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1130401
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG1204.8183656E-429.335791
CCCGTAA1651.02760765E-425.6021441
GTCGAGA1701.2586162E-424.8491382
AAAGTGC20450.022.3784034
TCGGCGG1600.002579080422.0018423
GACTTGT8600.020.4668313
CGGAGGT8750.020.115977
ACTTGTG9850.020.013864
CTACTCG6750.019.8179572
AAGTGCT22050.019.796675
CCAAAGT23050.019.243262
TACTCGG8650.018.7206423
CTACTAG2651.1708861E-418.5977842
GAGACTT10250.018.5459441
AGGCGAA3600.0087705217.80418145
CCCAAAG27550.017.6334191
CTACTTG9650.017.5102752
AGGCGGA14950.017.4248714
GCTACTT11450.017.2171631
TTGGGAG28250.017.1964846
Produced by FastQC (version 0.11.5)