FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314859

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314859
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences843128
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAA502.6138686E-454.9972761
CGGGTAA1800.003336424732.773678145
CTACTCG2952.910383E-1127.9647142
TACTCGG3850.026.7843863
GCTACTC7450.023.0693241
GTTAGTA2055.2472827E-420.1209531
CCGGACG1800.00585634619.0962759
GCTACTT6950.018.7940311
GCTCTAT3402.5176687E-618.1976261
GCTATAA1950.00929223817.627331
CCTAGTT2350.0013141117.552321
ATAGTAG2400.001513164617.1866476
CCTATAT3654.952748E-616.9512141
GCTAAGT2852.4509736E-416.8851281
TCGGGAG7350.016.83596
CCTAGCA3705.6373756E-616.7221451
GAGACCG2902.7987923E-416.5940065
TATATGC2500.001988337116.4991823
GCTATAT2953.1883793E-416.312751
GAGACTT6851.6370905E-1116.0575981
Produced by FastQC (version 0.11.5)