FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314860

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314860
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences706950
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCGGA2050.006963894728.1945145
GTCGAGA1350.001087633325.5190541
CGAGACT5600.023.3772751
TGATCAA6400.022.6082889
CTACTCG2803.8237522E-722.1468932
GAGACTT7200.022.0101831
GACTTGT6650.021.758353
CCTAAAT2809.450985E-619.6861271
TCGGCCA3700.005142403319.526596145
AGACTTG7900.019.1877422
AGTAGCT7600.019.0385575
CTTGTGA8050.018.8302085
ACTTGTG8050.018.8302084
GCTACTC7100.018.4384141
GCGGGAT1900.00789634918.131969
ACGGGGG3152.6066753E-517.498787
GATAAAT3955.3211625E-717.4434011
CCCTAAC2802.1040044E-417.225361
GCTACTT5602.6011548E-1017.225361
GTGATCA8600.016.824778
Produced by FastQC (version 0.11.5)