FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314861

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314861
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences706950
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTTG750.00475918957.768288145
GCTACGT900.00464904630.6294331
CTACTCG3200.030.1508462
CGCTACA1205.458782E-428.7150942
TAGGGCG1000.00777891527.566495
AAAGTGC11750.024.6338844
CCTATTA2001.5773247E-524.120681
AAGTGCT13350.023.2301885
TACACGG1500.002006390422.9720763
CAAGCGG1550.002427556322.2310412
TACTCGG4158.731149E-1121.5882153
GTTAGGG2809.43349E-619.690356
GACTTGT4603.4924597E-1019.4763243
CCCTTAA2551.026423E-418.9181791
GCTGGGA25200.018.8699199
ACTTGTG5951.8189894E-1218.5320954
CCCAAAG14800.017.6947061
ATATAAG1950.00915987217.6708282
CAAAGTG14850.017.6351283
CGGGTGT2802.1005578E-417.2290579
Produced by FastQC (version 0.11.5)