FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314863

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314863
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences718595
Sequences flagged as poor quality0
Sequence length35-150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG4700.038.864432
CGGATAT800.002376082835.1274641
TACTCGG5600.030.1092513
GCTACTC11450.028.8382641
GCTACTT7150.028.4950051
CTACTTG7850.026.8490162
GGAGGCT28900.026.2544359
TCGGGAG8750.025.693236
CTCGGGA8250.025.5472475
CCAACGA1651.0421899E-425.5472431
ACTCGGG7150.024.564664
CATGACG1500.001794022523.4183082
TTGGGAG19700.023.1805576
CATATAG2803.1703348E-722.5819383
AGTAGCT12600.021.1879925
GGGAGGC37900.021.1320888
TACTTGG9050.020.9600333
GTAGCTG13600.020.6632126
GCTGGGA31400.020.3605049
TAGCTGG15700.019.6892787
Produced by FastQC (version 0.11.5)