FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314867

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314867
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences979281
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAA951.1614281E-437.380425
CGATTAG951.1614281E-437.380423
CTACTCG4800.035.51142
TACTCGG5750.032.114663
AGACGTA1650.00545372929.644629145
ACTCGGG7000.028.4091194
CGAGTAG4200.025.3652843
GCTACTC12050.024.7548331
AAAGTGC18100.024.328254
AGTAGCT14300.023.3431575
CCGAGTA5200.023.2189922
GCTGGGA35550.022.575469
AAGTGCT19850.022.5412435
TCGGGAG8950.022.2194256
CCCGTAA1600.00245144922.1946261
TCCGCGG1953.0052793E-421.853173
CCAAAGT21300.021.3401832
CCCGAGT7400.021.1148851
GAGTAGC9850.020.9102654
GTAGCTG16100.020.7333646
Produced by FastQC (version 0.11.5)