FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005316110

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005316110
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences335132409
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAACAACCGATCTCGTATG3353490.10006462848539367TruSeq Adapter, Index 1 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1717050.034.1836931
TCGGAAG1798700.032.8006482
CGGAAGA1916800.030.766143
AGAGCAC3749750.016.1727757
AGCACAC3997450.015.2300399
TATGCCG846750.014.59028345-49
GAGCACA4177000.014.5619088
ACCGATC758600.014.45033335-39
GAAGAGC4323850.014.1803255
CTCGTAT886100.013.81030240-44
AACCGAT819650.013.42355635-39
CAACCGA931650.012.98161935-39
TCTCGTA858300.012.94503340-44
CCGATCT859450.012.86434535-39
ATGCCGT992000.012.37794645-49
CGTATGC1039650.011.876183545-49
ACAACCG1063950.011.33609930-34
TGCCGTC1094750.010.98306845-49
GCCGTCT1095350.010.93765850-54
ATCTCGT1049250.010.69560740-44
Produced by FastQC (version 0.11.5)