FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005316151

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005316151
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences316042522
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCGTAAGAGTGTAGATCTC3578500.11322843449527971Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG1860400.030.6990499
GAGCGTC2259400.025.4162488
AGAGCGT2485900.023.17317
ATCGGAA2681300.021.8224961
TCGGAAG2748850.021.2653372
AAGAGCG2911500.020.1284836
CGGAAGA2934850.019.972053
CGCCGTA691250.017.12009855-59
TCGCCGT782650.015.15595855-59
GCCGTAT850900.014.03230255-59
GTCGCCG948750.012.81577955-59
CCGTATC948850.012.21098355-59
GAAGAGC5079050.011.98969655
CGTATCA1001250.011.97667360-64
TGGTCGC1030650.011.97034550-54
GTGGTCG1089450.011.40278150-54
GTCCGTA1082750.011.08656830-34
GGTCGCC1127700.010.64067350-54
CCGTAAG1200500.010.27200930-34
TCTCGGT1295900.09.760406545-49
Produced by FastQC (version 0.11.5)