FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005316152

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005316152
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences306946910
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTGTGATATCTCGTATG3264710.10636073840912749TruSeq Adapter, Index 12 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA2007300.027.6850221
TCGGAAG2060050.027.0317882
CGGAAGA2188050.025.4281863
AGAGCAC3831700.014.9651797
AGCACAC3979650.014.5563769
TATGCCG824150.014.52194545-49
GAGCACA4145300.013.97468958
GAAGAGC4296700.013.4906955
CTCGTAT896050.013.43585240-44
TCTCGTA882450.012.75891740-44
CGTATGC928400.012.62732745-49
ATGCCGT999750.011.9944645-49
TATCTCG931100.011.81818340-44
TCGTATG1021850.011.44830540-44
TGCCGTC1036650.011.14649345-49
GCCGTCT1051650.010.96554250-54
ATCTCGT1063950.010.64505440-44
AAGAGCA5852800.010.1392316
GTCACCG1236850.09.8131425-29
CGTCTTC1250200.09.66942450-54
Produced by FastQC (version 0.11.5)