FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005316159

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005316159
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences323140980
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG1584400.032.747319
GAGCGTC1915600.027.2140888
AGAGCGT2141450.024.4860787
ATCGGAA2277200.024.0441881
TCGGAAG2371650.023.1367032
CGGAAGA2526450.021.7583163
AAGAGCG2564100.021.4478266
CGCCGTA658850.015.87334655-59
TCGCCGT734800.014.13004755-59
GCCGTAT815850.012.99288455-59
GTCGCCG887100.012.57367655-59
GAAGAGC4790550.011.8990315
TGGTCGC964450.011.55345150-54
CCGTATC915800.011.32153255-59
CGGTGTA1016250.011.17417335-39
CGTATCA965300.011.00836660-64
GTGGTCG1040550.010.8116150-54
GGTCGCC1040250.010.62934850-54
CATCGGT1059600.010.59660235-39
ATCGGTG1096700.010.32803335-39
Produced by FastQC (version 0.11.5)