FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005316178

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005316178
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences320037581
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA2547100.014.8286821
TCGGAAG2598650.014.5701662
CGGAAGA2681650.014.1476693
TATGCCG725300.011.45303545-49
CTCGTAT802150.010.3454940-44
GCGAATC730450.010.08550635-39
CGTATGC859900.09.64005945-49
TCTCGTA786450.09.545485540-44
AGAGCAC4206050.09.4307367
ATGCCGT896950.09.33077245-49
AGCACAC4366250.09.1029259
CGAATCT851800.08.85972235-39
GAGCACA4547350.08.8105258
TGCCGTC956900.08.3885945-49
GCCGTCT982050.08.33082750-54
GAAGAGC4859100.08.2378555
TCGTATG998550.08.17565940-44
AGCGAAT938000.08.0810835-39
ATCTCGT975600.07.80179640-44
CGTCTTC1191000.07.19184650-54
Produced by FastQC (version 0.11.5)