FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005316179

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005316179
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences320037581
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG1728900.020.9617429
GAGCGTC2090900.017.5047688
AGAGCGT2329300.015.8135337
CGCCGTA533950.014.29394355-59
ATCGGAA2611150.014.2526881
TCGGAAG2653200.014.0532742
AAGAGCG2743550.013.6340836
CGGAAGA2769400.013.4737893
TCGCCGT644600.011.88076255-59
GCCGTAT693400.011.22019755-59
GTCGCCG767000.010.34760555-59
TGGTCGC860300.09.42221450-54
CGTATCA839150.09.31470960-64
CCGTATC802700.09.29335955-59
GTGGTCG920700.08.87650550-54
GGTCGCC936700.08.34116750-54
GAAGAGC4928700.08.0381595
GTTCGCT1053450.07.50370530-34
TCTCGGT1135200.07.441237445-49
TGTTCGC1069350.07.05773825-29
Produced by FastQC (version 0.11.5)