FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005316197

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005316197
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences315618131
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTTGGACGTGTGTAGATCTC6521790.20663546733948562Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG2420000.042.841229
GAGCGTC2810650.037.0444158
AGAGCGT3067100.034.0815967
ATCGGAA3248150.032.309971
TCGGAAG3299950.031.7872962
CGGAAGA3454650.030.3901023
AAGAGCG3480100.030.2012426
CGCCGTA1108150.021.40204855-59
TCGCCGT1190050.019.859755-59
GAAGAGC5587700.019.1278365
GCCGTAT1263050.018.87718255-59
GTCGCCG1375100.017.29574655-59
CGTATCA1407350.016.68653560-64
TGGTCGC1483350.016.39478750-54
GTGGTCG1514700.016.1282250-54
CCGTATC1360150.015.32823555-59
TTGGACG1650050.014.66181830-34
TCTCGGT1727150.014.32230545-49
GACGTGT1741300.013.74519235-39
GGTCGCC1567850.013.62072550-54
Produced by FastQC (version 0.11.5)