FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005316205

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005316205
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences331155587
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAATGTGGGTGTAGATCTC6059970.18299464776959962Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG1963150.050.653789
GAGCGTC2340750.042.650158
ATCGGAA2613100.039.004011
AGAGCGT2584200.038.8752947
TCGGAAG2710900.037.5790022
CGGAAGA2909500.035.1442643
AAGAGCG3006150.033.9842036
GCGTAGA508250.024.1095289
GGCGTAG568050.023.3569728
GGTGTAG3013800.023.2797878
GGGCGTA471550.022.4079577
GGGTGTA2987700.020.5208957
GAAGAGC5155150.020.2212415
GTGTAGA3642900.017.9251489
CGCCGTA1253850.017.66100155-59
TCGCCGT1381600.016.03321655-59
GCCGTAT1401400.015.88529155-59
GGGGGTA1491100.015.0560767
GGGGTAG1864550.014.6572448
CGTATCA1542500.014.33608360-64
Produced by FastQC (version 0.11.5)