FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005316221

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005316221
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences345265125
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG1645250.017.4593459
GAGCGTC2023450.014.37830458
AGAGCGT2253900.013.0190747
CGCCGTA508400.012.09221555-59
ATCGGAA2433800.012.0149371
TCGGAAG2519050.011.6174892
AAGAGCG2641400.011.2335026
CGGAAGA2664450.011.0650813
TCGCCGT616950.010.20658655-59
GCCGTAT692750.09.18811755-59
TCGACGT719650.08.63296630-34
GTCGACG750800.08.19758530-34
CGACGGT147000.08.1913151
GTCGCCG786800.08.18375655-59
CGACGTT800300.07.877063330-34
CGTATCA836600.07.76363960-64
TGGTCGC851850.07.73071350-54
CCGTATC791000.07.497212455-59
GTGGTCG909600.07.36095750-54
GACGTTA955200.06.81663530-34
Produced by FastQC (version 0.11.5)