FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005316370

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005316370
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102223
Sequences flagged as poor quality0
Sequence length35-251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2610.2553241442728153No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGAT500.00916742132.82297245
TGTGTAC850.001492765677.27257240-244
TTAGAGT400.00278241166.117624
TACATGC400.00278241166.117622
ACATGCT400.00278241166.117623
CGAGCCC400.00278241166.117625
GTAAATT1600.003502641662.260765245
AACATGA1650.003956699760.37407245
GCTGAGC601.8751089E-458.7712259
CATTTAT757.96454E-658.7712251
TAAGACT450.004436778458.771229
TAATGAG450.004436778458.771227
ACCCCAA1100.00508255556.73202230-234
CTAGCCT1403.8198777E-1156.6722534
ATTCCGA1800.005583827455.342903245
ATATAGA801.1674312E-555.098026
GCCTTTC1007.043436E-752.8941047
GTACATG500.00673185152.8941041
CCCACGA500.00673185152.8941049
AGCCTTT1007.043436E-752.8941046
Produced by FastQC (version 0.11.5)