FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005316374

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005316374
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73296
Sequences flagged as poor quality0
Sequence length35-251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2790.3806483300589391No Hit
CATATAACCTCCCCCAAAATTCAGAATAATAACACACCCGACCACACCGC960.13097576948264572No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCTGG100.0048366752164.686311
GTTATTC100.0048366752164.686314
TGCCCAA751.2283665E-4144.4096245
TTCAGAG550.0094604185131.28146245
TTGAGTG704.5855533E-4103.87759240-244
GATGTAT400.001239801181.01146220-224
AATACGG700.00272368866.39599165-169
GTACATG400.003641955661.7573661
TACATGC400.003641955661.7573662
TAAGACT400.003641955661.7573669
TGATGTA1100.00443818258.65393220-224
ACTAGCA500.00880591749.405892
TGTGGGT2200.00881057349.23055245
GGAATTA1150.00944740148.47295195-199
GTACCGT1058.6850065E-539.2110256
GGTACCG1058.6850065E-539.2110255
TACCGTG1058.6850065E-539.2110257
GTGGGCC650.00342850233.086834220-224
CCGTGCA1252.4216539E-432.937269
CACTGAA1000.003238802332.937269
Produced by FastQC (version 0.11.5)