FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005316375

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005316375
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73296
Sequences flagged as poor quality0
Sequence length35-251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2790.3806483300589391No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAGCAT350.0022392946212.51675245
TGCGATG350.0022392946212.51675245
TGTGCAT400.0033392843185.95216245
TGCTGGG450.004749823165.2908245
CCATAGC100.0048036166165.064389
TCACCTA150.007701511140.9321785-89
CTTGCAT453.7200988E-4109.59254175-179
TGCGTAT408.819386E-488.25464235-239
AACGGGG550.001052618684.3718165-169
TTGCGTA1100.00317568163.8113235-239
ATAGGGA655.0255385E-663.4863053
CGGGTTC400.003609022861.8991436
GTATTAT400.003609022861.8991431
TACGGGT400.003609022861.8991434
GCCATCG400.003609022861.8991439
GTATACG400.003609022861.8991431
TATACGG400.003609022861.8991432
AAGGTTA1100.00361308861.772213230-234
AGGTTGT1100.00462079458.061516220-224
TTGTCTC250.006575438753.21914225-229
Produced by FastQC (version 0.11.5)