EGAF00005316376 FastQC Report

Basic Statistics

Measure	Value
Filename	EGAF00005316376
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	41313
Sequences flagged as poor quality	0
Sequence length	35-251
%GC	45

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	150	0.3630818386464309	No Hit
GTGCCTGTAAGTCCAGAAATGGTGTGTGGTTAGGAGTCAGGAGCAGAGAA	114	0.2759421973712875	No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT	76	0.18396146491419166	No Hit
GTATATACTCACACACACACACAAATCAAGGAGCACTTTGTACGTGTGTG	74	0.17912037373223924	No Hit
CTCAGAAACATTGTTTACCTGTCCCTTTTGCAAGCATAAGAGGACTTGTG	70	0.16943819136833443	No Hit
AGTATATACAACATACAAAGGAGTAAGAGAGGCCAGGAAACATGCCAGGA	64	0.15491491782247718	No Hit
GGTTAAGCCAGTGGATGGTGACACCATCGAATAAATAACCTACAGCCAGC	64	0.15491491782247718	No Hit
CTCTTACTCCTTTGTATGTTGTATATACTCACACACACACACAAATCAAG	61	0.14765328104954856	No Hit
AGTTAACAGTGACCAGGAGGACAACCTGCTTATTGATTCACTCAGAACTA	58	0.14039164427661993	No Hit
TTGTTTACCTGTCCCTTTTGCAAGCATAAGAGGACTTGTGAAGATGTACT	57	0.13797109868564375	No Hit
CCCTAGTACCTGCCAGCACATACCTTTACTTCCTTACATACACACACGTA	56	0.13555055309466754	No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA	55	0.13313000750369133	No Hit
CTCTAACATCCTGTATCCCTAGTACCTGCCAGCACATACCTTTACTTCCT	53	0.12828891632173892	No Hit
CTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTC	52	0.1258683707307627	No Hit
ATATAATATATGGTACTAAGTGCTATGGGGAAAAATAAAACCAGAAACAG	50	0.1210272795488103	No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT	44	0.10650400600295305	TruSeq Adapter, Index 1 (95% over 21bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGGTT	15	2.4747872E-4	442.676	245
GTAATGT	30	0.0019732283	221.338	245
TGTTATT	35	0.003129927	189.71828	245
GATGCGG	25	0.0044729356	168.66351	230-234
CTTGCGT	30	0.0067309197	147.1004	240-244
CTTAAGA	25	4.5678846E-4	104.10184	2
ACTTAAG	30	9.4286515E-4	86.75152	1
AGATGTT	30	9.4286515E-4	86.75152	5
AAGTCCA	55	1.3747176E-6	78.86502	9
GCCTGTA	60	2.3057783E-6	72.29293	3
ACAAGGT	15	0.006119001	54.194885	140-144
AGAGTGG	50	0.0071430807	52.05092	8
TCATAGA	50	0.0071430807	52.05092	6
AGTAGTT	195	0.0075423555	51.078	245
GTAAGTC	85	1.8188193E-5	51.03031	7
CTGTAAG	90	2.5503803E-5	48.195293	5
CCTGTAA	95	3.5104727E-5	45.658695	4
TAAGTCC	100	4.7522357E-5	43.375767	8
TGTAAGT	125	1.7690184E-4	34.70061	6
GTGCCTG	125	1.7690184E-4	34.70061	1