FastQCFastQC Report
Wed 8 May 2024
EGAF00005316379

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005316379
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59160
Total Bases10.5 Mbp
Sequences flagged as poor quality0
Sequence length35-251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2260.3820148749154834No Hit
CTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTC880.14874915483434753No Hit
GTACAAGGAAGGGGTAGGCTATGTGTTTTGTCAGGGGGTTGAGAATGAGT850.14367816091954022No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA840.14198782961460446No Hit
CTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTCGG780.13184584178498987No Hit
CTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATA760.12846517917511832No Hit
GGTTTATAGATAGTTGGGTGGTTGGTGTAAATGAGTGAGGCAGGAGTCCG750.12677484787018256No Hit
GTATAATACGCCTCACACTCATTCTCAACCCCCTGACAAAACACATAGCC720.12170385395537525No Hit
CTATTAACCTACTGGGAGAACTCTCTGTGCTAGTAACCACGTTCTCCTGA710.12001352265043949No Hit
GTATTATACCATAGCCGCCTAGTTTTAAGAGTACTGCGGCAAGTACTATT700.11832319134550372No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTAAGTAGGAGAGTGATAT660.11156186612576065No Hit
GTATAGGATTGCTTGAATGGCTGCTGTGTTGGCATCTGCTCGGGCGTATC640.10818120351588911No Hit
CTACTATAACCACCCTAACCCTGACTTCCCTAATTCCCCCCATCCTTACC620.10480054090601758No Hit
CTATTGATCCCCACCTCCAAATATCTCATCAACAACCGACTAATCACCAC620.10480054090601758No Hit
TCCTAATACTAACTACCTGACTCCTACCCCTCACAATCATGGCAAGCCAA620.10480054090601758No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC610.1031102096010818No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)