FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005316382

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005316382
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62592
Sequences flagged as poor quality0
Sequence length35-251
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2920.4665132924335378No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT1180.18852249488752557No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT860.13739775051124745RNA PCR Primer, Index 27 (95% over 23bp)
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA650.10384713701431492No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGGCC300.00588532153.99709240-244
TTTTCAA450.0065026544148.79889245
TGCCCGG300.009963212129.17703205-209
TTATACA402.3159009E-7106.477462
TAGTGCT350.001874002273.0131157
CTTGGTT400.003182057363.886489
GTTAAGC400.003182057363.886483
TTCTTGA400.003182057363.886487
ATAGTGC400.003182057363.886486
TATACAC706.455286E-660.844263
TCTTGAT450.005073277356.787988
GAAGAGT450.005073277356.787984
CATCTCC956.2252184E-753.7991379
CTGTAGC653.289161E-452.4196741
AATGGCC500.007696432551.109183
AGCTACA500.007696432551.109187
TACACAT852.0385789E-550.107045
CTTATAC1205.4420525E-849.689481
ATTGTTC704.741291E-448.6754078
GTGCTTG704.741291E-448.6754079
Produced by FastQC (version 0.11.5)