FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005316383

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005316383
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62592
Sequences flagged as poor quality0
Sequence length35-251
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2910.46491564417177916No Hit
CTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTC680.108640081799591No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAG200.0022218158213.30281225-229
TGAGAAC200.0029942333193.09927205-209
TCACGCT150.0030725475191.52936155-159
ACCATGG100.0043422924170.710956
TGTGAAT1700.00435106658.829906245
GTGCTCA450.005032581756.903659
CAAGATA450.005032581756.903654
CTAACTA500.00763476751.2132879
CAATGCC704.6939473E-448.7745559
TCTATAC756.595487E-445.5229233
AAGATAG850.001221297740.1672825
ATACAAT850.001221297740.1672826
TAATACA1050.003445475632.5163734
TACAATG1050.003445475632.5163737
AATACAA1150.00537751429.688865
ATATAGG1200.006620547328.4518243
GCTAATA1200.006620547328.4518242
CTTATTA1250.00808040927.3137511
ACAATGC1250.00808040927.3137518
TGCTAAT1250.00808040927.3137511
Produced by FastQC (version 0.11.5)