FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005316384

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005316384
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70889
Sequences flagged as poor quality0
Sequence length35-251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2780.39216239472978887No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC720.10156723892282299No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT710.1001565828266727No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATAAG400.00543661158.07455245
CGGAATG450.0077317140.51071245
TGCGCTT701.5874617E-4135.49246245
CATAAGT350.009961692129.18277240-244
CTAGTGT802.70137E-4118.555916245
AGGAGTG350.001721531374.602365
CGAGCCC350.001721531374.602365
CCCACGA400.002923446565.277079
CTGCAGG400.002923446565.277076
TACTGCA450.004661420458.024064
GTACTTG1250.006039836454.256763240-244
CAGTATT500.007072312352.2216579
AACCATC1250.007369910451.600838230-234
AGGCCAT851.7972927E-551.1977045
CCATATG851.7972927E-551.1977048
TACGAGT704.2675436E-449.7349134
TAGGCCA1108.238251E-539.561864
ATCAGAG900.001471062538.682712
CAGGCCA900.001471062538.682719
ATACCTA950.001918535736.646783
Produced by FastQC (version 0.11.5)