FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005316385

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005316385
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70889
Sequences flagged as poor quality0
Sequence length35-251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2750.3879304264413379No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTTA155.8767102E-5174.356698
TCCCAAG100.0040774904174.356693
TCCCGGA400.0049249544163.37268245
GTACCGT201.8488574E-4130.767526
TACCGTT201.8488574E-4130.767527
TTACGTG552.8956338E-4116.65056230-234
CCTAACG709.432824E-486.68754220-224
ACTTGCG850.001349447979.191895240-244
TATATCG400.00290458665.383765
GATAGTC551.28587E-463.4024287
AAATGCG551.28587E-463.4024285
GGGTACT550.00421196159.513485235-239
TACGTGG550.00454687958.375957230-234
AATGCGT601.9773096E-458.1188966
ATAAATG601.9773096E-458.1188963
CCGTTAA500.0070267952.3070079
ACACCTT550.00705543252.24784210-214
CTAACGT600.00794868450.692593220-224
CCGCAGA250.00901259849.142155220-224
TAATACG755.9488195E-446.495124
Produced by FastQC (version 0.11.5)