FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005316386

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005316386
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72136
Sequences flagged as poor quality0
Sequence length35-251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2370.32854607962737054No Hit
CTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTC1090.15110347122102696No Hit
CTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTCGG950.13169568592658312No Hit
CTATTGATCCCCACCTCCAAATATCTCATCAACAACCGACTAATCACCAC930.128923145170234No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA920.12753687479205944No Hit
GTATAATACGCCTCACACTCATTCTCAACCCCCTGACAAAACACATAGCC910.12615060441388487No Hit
GTCTAGGCCATATGTGTTGGAGATTGAGACTAGTAGGGCTAGGCCCACCG880.12199179327936122No Hit
GTACAAGGAAGGGGTAGGCTATGTGTTTTGTCAGGGGGTTGAGAATGAGT870.12060552290118665No Hit
GTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAATATCT830.11506044138848842No Hit
CTACTATAACCACCCTAACCCTGACTTCCCTAATTCCCCCCATCCTTACC750.10397027836309194No Hit
CATTTACACCAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCC740.10258400798491737No Hit
CTATTAACCTACTGGGAGAACTCTCTGTGCTAGTAACCACGTTCTCCTGA740.10258400798491737No Hit
ACTATAACCACCCTAACCCTGACTTCCCTAATTCCCCCCATCCTTACCAC740.10258400798491737No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)