FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005316390

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005316390
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79549
Sequences flagged as poor quality0
Sequence length35-251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2520.3167858804007593No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG1030.12947994317967543No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAATCA300.0032452976187.95393245
GATGGGA300.0032452976187.95393245
GGTGGTC800.002904006365.34075215-219
TACATGC450.003637426861.78172
CACGGAG850.004378553558.911495205-209
GAGCATG652.1728197E-457.0292639
CAAACAG1500.00518397156.386177245
AGCTCAT350.00527662656.256607145-149
GTCATCA500.00552023455.6035359
GTACATG500.00552023455.6035351
ATCTAGG500.00552023455.6035351
GAGCTCC500.00552023455.6035359
GCATGGA500.00552023455.6035352
TCATCCA1550.005902199554.567272245
ATGAGCA703.1333134E-452.9557467
AGTCATC550.00804751850.5486648
ACCCATG550.00804751850.5486647
AGAGCTC550.00804751850.5486648
TCCTTAT858.161694E-443.6106152
CATTTAT858.161694E-443.6106151
Produced by FastQC (version 0.11.5)