FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005316391

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005316391
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79549
Sequences flagged as poor quality0
Sequence length35-251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2510.3155287935737721No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGGCA250.001731358231.84804245
CACAGGG300.0029879424193.2067245
TACATGG150.004388135170.10225135-139
TGCCTGG350.004738646165.60574245
CGTTACC250.006576034148.52309230-234
CCATGTT1200.00191900172.45251245
CTGAGAG1300.002635942366.87924245
CTATAAC500.00547032755.7312934
TTTAATG1352.5809015E-455.00855230-234
TCTAGTG550.00797484250.664812
TCTCCGT400.00928073248.771034240-244
ACCAGTC1200.00944483148.492588240-244
GTGAACC200.001197722740.37945660-64
ATCCAGT200.001290108539.7740155-59
TATCCAG200.001290108539.7740155-59
CGCCACT200.001369089439.29627650-54
CCAACGC200.001449035838.8452545-49
GCCAACG200.001449035838.8452545-49
GGCAAGC200.001559349438.2696940-44
CATGGCA200.001686792637.662735-39
Produced by FastQC (version 0.11.5)