FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005316394

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005316394
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39202
Sequences flagged as poor quality0
Sequence length35-251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTG1370.3494719657160349TruSeq Adapter, Index 12 (96% over 29bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN860.2193765624202847No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA680.17346053772766695No Hit
CTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGA640.163256976684863RNA PCR Primer, Index 12 (96% over 29bp)
GTACAAGGAAGGGGTAGGCTATGTGTTTTGTCAGGGGGTTGAGAATGAGT630.16070608642416204No Hit
CTATTGATCCCCACCTCCAAATATCTCATCAACAACCGACTAATCACCAC560.14284985459925514No Hit
GTATAATACGCCTCACACTCATTCTCAACCCCCTGACAAAACACATAGCC540.13774807407785317No Hit
CTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTC530.13519718381715218No Hit
GTCTAGGCCATATGTGTTGGAGATTGAGACTAGTAGGGCTAGGCCCACCG520.1326462935564512No Hit
CTATTAACCTACTGGGAGAACTCTCTGTGCTAGTAACCACGTTCTCCTGA510.13009540329575023No Hit
CTAGTATATCGCTCACACCTCATATCCTCCCTACTATGCCTAGAAGGAAT500.12754451303504924No Hit
GTATAGGATTGCTTGAATGGCTGCTGTGTTGGCATCTGCTCGGGCGTATC500.12754451303504924No Hit
CTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTCGG500.12754451303504924No Hit
CTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATA490.12499362277434824No Hit
TCCTAATACTAACTACCTGACTCCTACCCCTCACAATCATGGCAAGCCAA470.11989184225294627No Hit
CTACTATAACCACCCTAACCCTGACTTCCCTAATTCCCCCCATCCTTACC450.1147900617315443No Hit
TCTCTATACTAATCTCCCTACAAATCTCCTTAATTATAACATTCACAGCC450.1147900617315443No Hit
GTATTATACCATAGCCGCCTAGTTTTAAGAGTACTGCGGCAAGTACTATT450.1147900617315443No Hit
GGTTATAGTAGTGTGCATGGTTATTACTTTTATTTGGAGTTGCACCAAAA450.1147900617315443No Hit
GTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAATATCT450.1147900617315443No Hit
ACTATAACCACCCTAACCCTGACTTCCCTAATTCCCCCCATCCTTACCAC430.10968828121014235No Hit
GGTTAATAGTGGGGGGTAAGGCGAGGTTAGCGAGGCTTGCTAGAAGTCAT420.10713739094944136No Hit
ATACTAATCTCCCTACAAATCTCCTTAATTATAACATTCACAGCCACAGA410.10458650068874036No Hit
CCCTAGTAGGCTCCCTTCCCCTACTCATCGCACTGATTTACACTCACAAC410.10458650068874036No Hit
ACACTAAAGGACGAACCTGATCTCTTATACTAGTATCCTTAATCATTTTT400.10203561042803938No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC400.10203561042803938No Hit
TTATAGTAGTGTGCATGGTTATTACTTTTATTTGGAGTTGCACCAAAATT400.10203561042803938No Hit
GGTTTATAGATAGTTGGGTGGTTGGTGTAAATGAGTGAGGCAGGAGTCCG400.10203561042803938No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGAGT300.00285663195.71313245
CTCTAAA200.0032612318187.5157230-234
AACTGTC100.0035240278182.965527
TCTCCGA402.0776375E-591.482761
ACTAAGT350.001407909178.4137958
CGAGCCC350.001407909178.4137955
GGGGACA200.001512270577.077835180-184
ATATCTC400.002391419168.612078
CCTCCAA400.002391419168.612071
TATCTCA400.002391419168.612079
CTCCGAG551.0073817E-466.532912
CCCACGA450.00381398960.9885069
GAGCCCA652.30156E-456.2970856
ACTATTG500.005787931454.8896523
TCCGAGC500.005787931454.8896523
GTCCTAA500.005787931454.8896521
TCCTATG500.005787931454.8896527
CCCACAC500.005787931454.8896529
TTGACCC500.005787931454.8896527
CCGAGCC500.005787931454.8896524
Produced by FastQC (version 0.11.5)