FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005316396

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005316396
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72264
Sequences flagged as poor quality0
Sequence length35-251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2520.34872135503155094No Hit
GTTGTATATAGCCTAGAATTTTTCGTTCGGTAAGCATTAGGAATGCCATT980.1356138602900476No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG960.1328462304882099No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG930.12869478578545335No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA870.12039189637994023No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT850.11762426657810253No Hit
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG820.11347282187534596No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT790.10932137717258938No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTC790.10932137717258938No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC770.10655374737075168No Hit
ATGCTAGGGTGAGTGGTAGGAAGTTTTTTCATAGGAGGTGTATGAGTTGG730.10101848776707628No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTATT852.9558068E-4115.899925245
AGTGGTG954.600962E-4103.699936245
ATGTAGA400.00280900965.939242
ATATGGA1550.003213707863.55802245
CTTAGGT1250.007001010252.5413245
TTGTATA550.009905199547.955812
ACTACGC1202.5104982E-643.9594888
CTATAGA850.001056982741.3736423
AAGAACT850.001056982741.3736429
TTAGGTT950.001825992537.018525
TTGTCTG950.001825992537.018529
ATAGACG1201.2960038E-436.6329083
GGTTTGA1050.0029835733.4929478
ATATACA1050.0029835733.4929471
TATAGAG1100.003747210431.970547
CGAGCCT653.4534436E-431.031244225-229
CCTACCG653.9239926E-430.362759220-224
GGGTATA1555.818244E-428.3609621
TTAGCAA1250.007001010228.1340754
CCTATAG1250.007001010228.1340752
Produced by FastQC (version 0.11.5)