FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005316398

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005316398
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71032
Sequences flagged as poor quality0
Sequence length35-251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3420.4814731388669895No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT860.12107219281450614No Hit
CTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTCGG770.10840184705484851No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCATA350.003204142188.60422245
GAAGCGT100.0043136171.106234
AAGCGTG100.0043136171.106235
TTATACA351.50166925E-597.774992
CCGTCGG250.00750053287.22094235-239
TCACCGT852.6109285E-477.66056245
TCGGAAA200.003100906764.34712230-234
GAGGGCA400.003129482764.164849
CGTAGAT200.004080721660.04026220-224
TATACAC602.1700295E-457.035413
GATTCAT1150.00332410655.189674230-234
TGGCTAC200.006038907454.382206200-204
CGTCGGA250.00750053251.477695230-234
GTCGGAA250.00750053251.477695230-234
GGCTTAT500.00756955151.3318671
CTTATGC500.00756955151.3318673
CCTTCTG500.00756955151.3318679
AGATGCC450.001896079747.013428230-234
TATGCGG756.5268617E-445.628335
ACACATC1203.0279643E-642.7765586
Produced by FastQC (version 0.11.5)