FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005316402

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005316402
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62020
Sequences flagged as poor quality0
Sequence length35-251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1860.2999032570138665No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA970.15640116091583361No Hit
GTATAATACGCCTCACACTCATTCTCAACCCCCTGACAAAACACATAGCC850.13705256368913254No Hit
GTACAAGGAAGGGGTAGGCTATGTGTTTTGTCAGGGGGTTGAGAATGAGT770.12415349887133183No Hit
CTACTATAACCACCCTAACCCTGACTTCCCTAATTCCCCCCATCCTTACC750.12092873266688164No Hit
CTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTC720.11609158336020639No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTAAGTAGGAGAGTGATAT710.1144792002579813No Hit
GTATTATACCATAGCCGCCTAGTTTTAAGAGTACTGCGGCAAGTACTATT700.1128668171557562No Hit
ACTATAACCACCCTAACCCTGACTTCCCTAATTCCCCCCATCCTTACCAC670.10802966784908094No Hit
CCTATACCCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACT660.10641728474685586No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)