FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005316404

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005316404
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67394
Sequences flagged as poor quality0
Sequence length35-251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2300.34127667151378466No Hit
ATCCTATATATCTTAATGGCACATGCAGCGCAAGTAGGTCTACAAGACGC770.11425349437635397No Hit
ATCTTAGGGGCCATCAATTTCATCACAACAATTATCAATATAAAACCCCC770.11425349437635397No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT720.10683443629996736No Hit
TGCTAATACAATGCCAGTCAGGCCACCTACGGTGAAAAGAAAGATGAATC680.10089918983885807No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGTTG1158.080204E-6110.3163245
TATTCTG900.001847190373.1597230-234
TACCAAC850.001943299272.24406220-224
TTTCGGT950.001946555372.19135240-244
GCCCGTC350.003105053764.29741235-239
GTCCATA500.00740500451.61521
TTCGGTC700.00902006149.084187240-244
GTGTTCC808.726141E-443.012679
ATATCTT808.726141E-443.012675
CCATACA1253.8573708E-641.292162
CTGCCGG800.002518113739.644917245
ACGGTCG800.00774250139.644917245
ACTAAGT900.001557410338.2334828
TAACACT1201.4720524E-435.8438874
TAGGGGC1201.4720524E-435.8438875
GTGCTCA1602.0961143E-532.2595029
CATACAC2708.62201E-1031.8612373
ATATATC1100.00416671731.281943
GGGGCCA1100.00416671731.281947
CGGCGGC802.6660267E-531.17915220-224
Produced by FastQC (version 0.11.5)