FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005316405

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005316405
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67394
Sequences flagged as poor quality0
Sequence length35-251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2290.3397928598985073No Hit
ATCCTATATATCTTAATGGCACATGCAGCGCAAGTAGGTCTACAAGACGC800.11870492922218595No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT760.11276968276107664No Hit
GGTTTATAGATAGTTGGGTGGTTGGTGTAAATGAGTGAGGCAGGAGTCCG760.11276968276107664No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCATA450.00487168257.3777927
GGGCTAA500.00739094751.6400157
TTAGGGC808.7055203E-443.0333444
AGCCCTC1000.00260607234.4266785
AGGCCTG1000.00260607234.4266788
GACTCTT850.002942392133.593105225-229
AGTATGC1302.3494888E-433.1025738
CGGAAGC1050.00331012232.787316
ACGGAAG1050.00331012232.787315
GAAGCAA1100.004156926631.296988
CACGGAA1100.004156926631.296984
GCCATAC1100.004156926631.296988
GTATACT1100.004156926631.296984
TACTCAA1150.005166659629.9362415
TCAAAGC1200.00636119728.6888981
ACCTAAC1250.00776416927.5413428
TCCACGG1250.00776416927.5413422
TATAGAT1250.00776416927.5413425
CCACGGA1250.00776416927.5413423
TTATAGA1607.9278153E-426.895844
Produced by FastQC (version 0.11.5)