FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005316407

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005316407
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25834
Sequences flagged as poor quality0
Sequence length35-251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1050.40644112410002325No Hit
ATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA390.15096384609429433No Hit
ATATACACCAACAAACAATGTTCAACCAGTAACTACTACTAATCAACGCC350.13548037470000773No Hit
TTATGGTACCGTGCAATATTCATGGTGGCTGGCAGTAATGTACGAAATAC350.13548037470000773No Hit
ATATAGGCCTCGCCCGATGTGTAGGAAGAGGCAGATAAAGAATATTGAGG310.11999690330572114No Hit
CCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTTTTAAGCTGTG310.11999690330572114No Hit
GTTGTAGTCCGTGCGAGAATAATGATGTATGCTTTGTTTCTGTTGAGTGT300.11612603545714949No Hit
GTAATAGGGCAAGGACGCCTCCTAGTTTGTTAGGGACGGATCGGAGAATT290.11225516760857783No Hit
CCCTTAACAGTACATAGTACATAAAGCCATTTACCGTACATAGCACATTA290.11225516760857783No Hit
CTCTAACACTATGCTTAGGCGCTATCACTACTCTGTTCGCAGCAGTCTGC280.1083842997600062No Hit
CATATAACCTCCCCCAAAATTCAGAATAATAACACACCCGACCACACCGC280.1083842997600062No Hit
ATTATACCCTAGCCAACCCCTTAAACACCCCTCCCCACATCAAGCCCGAA260.1006425640628629No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATCTA200.003844348177.25210-214
AATTTGT100.0038899004176.901764
TAATTTG100.0038899004176.901763
GTAATTT100.0038899004176.901762
TCGTCAC250.0057826373154.58026225-229
CGTTATC250.0065603647148.21893220-224
TTGAGGG400.0066582668147.12907245
GTAATAA400.0066582668147.12907245
AATACCA1000.002252713335.3803565
TACACCA1150.00446821830.7655265
ATACACC1150.00446821830.7655264
CTACTAA1150.00446821830.7655261
Produced by FastQC (version 0.11.5)