FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005316409

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005316409
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40092
Sequences flagged as poor quality0
Sequence length35-251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1660.4140476903122818No Hit
CTACTATAACCACCCTAACCCTGACTTCCCTAATTCCCCCCATCCTTACC530.1321959493165719No Hit
CTATTAACCTACTGGGAGAACTCTCTGTGCTAGTAACCACGTTCTCCTGA530.1321959493165719No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA510.12720742292726728No Hit
CTATTGATCCCCACCTCCAAATATCTCATCAACAACCGACTAATCACCAC500.12471315973261497No Hit
GGTTAATAGTGGGGGGTAAGGCGAGGTTAGCGAGGCTTGCTAGAAGTCAT490.12221889653796268No Hit
CTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTC480.11972463334331039No Hit
GTCTAGGCCATATGTGTTGGAGATTGAGACTAGTAGGGCTAGGCCCACCG460.1147361069540058No Hit
TCCTAATACTAACTACCTGACTCCTACCCCTCACAATCATGGCAAGCCAA450.11224184375935348No Hit
GGTTTATAGATAGTTGGGTGGTTGGTGTAAATGAGTGAGGCAGGAGTCCG450.11224184375935348No Hit
GTACAAGGAAGGGGTAGGCTATGTGTTTTGTCAGGGGGTTGAGAATGAGT420.10475905417539659No Hit
GTATTATACCATAGCCGCCTAGTTTTAAGAGTACTGCGGCAAGTACTATT420.10475905417539659No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTAAGTAGGAGAGTGATAT410.1022647909807443No Hit
ACTATAACCACCCTAACCCTGACTTCCCTAATTCCCCCCATCCTTACCAC410.1022647909807443No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)