FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005316411

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005316411
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64056
Sequences flagged as poor quality0
Sequence length35-251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1430.22324216310728112No Hit
CTCTAACACTATGCTTAGGCGCTATCACCACTCTGTTCGCAGCAGTCTGC660.10303484451105283No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAAAA250.0026327528201.5482245
GTTTCCG100.0027898457197.920968
TGACATT350.007202939143.96301245
CAAGGGA703.93251E-4107.97225245
TACCGCA550.009624951591.61282245
CTGTACA400.001756185374.220369
ACTGTAC400.001756185374.220368
TATATCG450.002801773465.9736565
CGGTGCT450.002801773465.9736569
ATTCCAT500.004253200359.376291
TATAGAG858.393155E-658.2120482
GCAAGGG200.006029747454.404144240-244
CGCAAGG200.006029747454.404144240-244
AAAACGC550.006202113353.9784475
GAGACAA550.006202113353.9784479
CCACTCT855.897784E-446.5696374
GTGAGGT855.897784E-446.5696374
GTACTGT1100.002096386935.985636
ATAGAGG1150.00260723734.4210363
CTAACAC1552.9056886E-431.9227373
Produced by FastQC (version 0.11.5)