FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005316412

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005316412
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43962
Sequences flagged as poor quality0
Sequence length35-251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN940.21382102725080754No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA610.1387561985350985No Hit
GTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAATATCT600.13648150675583456No Hit
ACCATACACAACACTAAAGGACGAACCTGATCTCTTATACTAGTATCCTT540.12283335608025113No Hit
CTACTATAACCACCCTAACCCTGACTTCCCTAATTCCCCCCATCCTTACC490.11145989718393157No Hit
GTACAAGGAAGGGGTAGGCTATGTGTTTTGTCAGGGGGTTGAGAATGAGT480.10918520540466768No Hit
CTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTCGG450.10236113006687594No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)