FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005316413

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005316413
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43962
Sequences flagged as poor quality0
Sequence length35-251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN940.21382102725080754No Hit
CTATTGATCCCCACCTCCAAATATCTCATCAACAACCGACTAATCACCAC520.1182839725217233No Hit
GTATTATACCATAGCCGCCTAGTTTTAAGAGTACTGCGGCAAGTACTATT450.10236113006687594No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTAG155.760056E-4334.50146245
AGGTACT100.002849236196.462334
ATTTAGT150.009579679130.974888
TAGGTAC150.009579679130.974883
GCTATAC150.009579679130.974883
TTCTTTG150.009579679130.974882
GTACTGT150.009579679130.974886
ACTGTTT150.009579679130.974888
CCTTCTG252.7873705E-4117.8773969
CAATGTA350.001062158884.198145
AATGTAT350.001062158884.198146
CCCAATG400.001804675473.673373
TATACCT400.001804675473.673375
TCCCAAT400.001804675473.673372
GATCCCC601.0909831E-465.487446
ATGTATG450.002879063465.487447
TTATATG450.002879063465.487442
ACTCACT450.002879063465.487448
ATTGATC601.0909831E-465.487443
ATACCTT450.002879063465.487446
Produced by FastQC (version 0.11.5)