FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005316414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005316414
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69218
Sequences flagged as poor quality0
Sequence length35-251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2780.4016296339102545No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA960.13869224768123897No Hit
CTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTC840.12135571672108411No Hit
CTACTATAACCACCCTAACCCTGACTTCCCTAATTCCCCCCATCCTTACC820.11846629489439163No Hit
CTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTCGG810.11702158398104538No Hit
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTG780.11268745124100668TruSeq Adapter, Index 12 (96% over 29bp)
CTATTAACCTACTGGGAGAACTCTCTGTGCTAGTAACCACGTTCTCCTGA780.11268745124100668No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT750.10835331850096795No Hit
GTATTATACCATAGCCGCCTAGTTTTAAGAGTACTGCGGCAAGTACTATT720.10401918576092924No Hit
GTATAATACGCCTCACACTCATTCTCAACCCCCTGACAAAACACATAGCC710.102574474847583No Hit
GTGCCTGTAAGTCCAGAAATGGTGTGTGGTTAGGAGTCAGGAGCAGAGAA710.102574474847583No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)