FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005316415

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005316415
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69218
Sequences flagged as poor quality0
Sequence length35-251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2780.4016296339102545No Hit
CTATTAACCTACTGGGAGAACTCTCTGTGCTAGTAACCACGTTCTCCTGA870.12568984946112283No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA850.12280042763443036No Hit
GTACAAGGAAGGGGTAGGCTATGTGTTTTGTCAGGGGGTTGAGAATGAGT710.102574474847583No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGTT259.0524036E-4287.53723245
ATCCTTG300.005993904153.07307225-229
AGTAACG300.008024597138.86743210-214
GTCCATC501.2731252E-679.987631
TAGCTTA1350.001293323679.87146245
ATCGAAA900.001542654976.53654225-229
ACACATC350.002406274268.560848
GGATAGA250.00360572361.933174240-244
ATATAGC400.00408461159.990736
ATTGACT250.004393906358.91946235-239
GCAACTG500.005228413356.35337190-194
AACCATC1300.00583777354.699768230-234
TGTACTA1053.126297E-853.325095
CATTCAA2100.00744911351.345936245
TCCTTGT1104.5109118E-850.9012221
TCATAGA802.0687568E-549.9922756
AAGGGTG500.00987342647.992586
CTTGTAC1208.949064E-846.6594543
TCACACA1051.9183353E-645.707229
TTGTACT1301.6791091E-743.0702674
Produced by FastQC (version 0.11.5)