FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317839

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317839
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3773354
Sequences flagged as poor quality0
Sequence length35-76
%GC63

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA288450.7644392760393008No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN255620.677434452214131No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC157220.41665849533332944No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC124590.3301837039408442No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC73130.1938063590111079No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA70250.18617389198045028No Hit
CGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA66510.1762622854892491No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT61200.16218992440147414No Hit
GGGGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGT56650.15013168655790046No Hit
GGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA49150.13025547033222962No Hit
GGGGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTC40310.10682803680757225No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGACT56900.054.3257261
GCGCGGT82100.053.332129
CCGAACT9950.052.75692467
ACTCTTC75700.051.254285
GGACTCT75300.050.5390243
GGGACTC71150.050.2002142
TCTGGTC74800.050.14999410
CGCGGTG87550.049.9747810
CGGGACT16850.049.4991951
TTCTGGT76500.049.3445859
GCCGGTT10500.049.1001973
TGGCGCG90900.048.0957215
CTCTTCT81100.047.75796
TCTTCTG80600.047.3381927
CGGTTCA11100.046.748585
CTTAGGC10850.046.6650818
CTGGTCC79600.046.61518511
GACTCTT87500.045.195014
TGTAGTC98350.045.0125626
GTAGTCC98900.044.71196727
Produced by FastQC (version 0.11.5)