FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317845

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317845
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3894082
Sequences flagged as poor quality0
Sequence length35-76
%GC63

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA316420.8125663506829081No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN252520.6484711929538206No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC162680.41776213238447474No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC131920.3387704727327262No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC79710.20469522727050946No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA73920.18982651110069074No Hit
CGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA71840.18448507247664533No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT62730.16109059850306182No Hit
GGGGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGT59620.15310412056037853No Hit
GGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA55520.14257532327259673No Hit
GGGGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTC42670.1095765317730854No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG39990.10269429354594999No Hit
GGGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTC39330.10099941398255097No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGACT57750.053.3275641
GCGCGGT80100.052.3258139
ACTCTTC75350.051.8416985
CCGAACT11200.051.7317667
GGACTCT74750.051.5734523
GCCGGTT11150.050.827733
CGGTTCA11350.050.234325
GGGACTC72600.049.6884842
TCTGGTC76150.049.55830410
CGGGACT15300.049.2080461
CGCGGTG85300.049.05638510
TCTTCTG78400.049.002427
CTCTTCT81200.047.8560266
TTCTGGT79700.047.6063659
TGGCGCG88200.047.5576315
CTTAGGC12450.045.87601518
CTGGTCC81150.045.8356711
ACCATAT12450.045.53165455
CCGGTTC12550.045.4281044
CGGTATA752.0008883E-1145.4228131
Produced by FastQC (version 0.11.5)