FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317847

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317847
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3525297
Sequences flagged as poor quality0
Sequence length35-76
%GC64

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA205340.5824757460151584No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC171440.4863136354185194No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC132480.37579812424314885No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT91190.2586732408645286No Hit
GGGGGGCCCTTCCCGTGGATCGCCCCAGCTGCGGCGGGCGTCGCGGCCGC56520.16032691713634342No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC53750.15246942314363868No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG47220.1339461611319557No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN43640.12379098839048171No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC43320.12288326345269633No Hit
CGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA40790.11570656316333063No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG40150.11389111328775987No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC39610.11235932745524704No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGG11750.057.4579735
GTCTACG11700.057.409124
CCGAACT13700.056.734167
CGGTTCA13800.056.161095
TACGGCC12200.055.6193857
GCCGGTT14150.054.2927973
GCGCGGT74750.053.6842469
GCCATAC12800.052.47477311
CTACGGC13100.051.536736
CGAACTT15650.051.0443168
ACCATAT15600.050.715955
GGGGACT44150.050.5972631
ACTCTTC45250.050.3170285
CGCGGTG80300.050.10392810
CTTAGGC15550.050.0807218
TGGCGCG81800.049.5220315
CATATTG15900.048.98516557
CCATATT16000.048.8375456
TTAGGCA15950.048.83518619
CACCATA16400.048.19025454