FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317849

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317849
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3528040
Sequences flagged as poor quality0
Sequence length35-76
%GC64

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA207960.5894490992165622No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC179800.5096314100747157No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC138830.3935046087912835No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT90970.2578485504699493No Hit
GGGGGGCCCTTCCCGTGGATCGCCCCAGCTGCGGCGGGCGTCGCGGCCGC58830.1667498100928561No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC55010.15592226845500617No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG47030.13330347728483805No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC44860.1271527533701432No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG43620.12363805399031758No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN41760.11836600492057914No Hit
CGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA41300.11706216482806318No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC40930.11601342388408295No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA252.2708045E-456.59103860
GTCTACG10150.056.3476754
CCGAACT13100.054.37135767
TCTACGG10600.053.9555555
CGGTTCA13200.053.50745
TACGGCC10650.053.378737
GCGCGGT81850.053.122029
GGGGACT42350.050.111521
GCCGGTT14100.050.0920373
CGCGGTG87000.049.9350110
ACTCTTC44300.049.9304245
TTAGGCA14250.049.59219419
TGGCGCG87100.049.40513215
TCTGGTC44850.048.2399110
CGAACTT15450.047.89925868
GGACTCT49350.047.892873
CTACGGC11950.047.8601576
CTTAGGC14700.047.5976218
TTCTGGT45950.047.4626669
GGGTCTA10500.047.2572862
Produced by FastQC (version 0.11.5)