FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317851

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317851
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3629833
Sequences flagged as poor quality0
Sequence length35-76
%GC64

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA215610.5939942691578373No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC181810.5008770375937406No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC140570.3872629952948249No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT92310.2543092202864429No Hit
GGGGGGCCCTTCCCGTGGATCGCCCCAGCTGCGGCGGGCGTCGCGGCCGC58820.16204602250296365No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC56060.15444236690778887No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG48840.13455164466244038No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC44950.12383489818953103No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN43190.1189861902737674No Hit
CGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA42720.11769136486444416No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG42480.11703017742138551No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC41190.11347629491494512No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGG11400.056.199515
CCGAACT15300.055.93651667
TACGGCC11550.055.182847
GTCTACG11850.054.0653534
CGGTTCA15700.053.9709325
GCGCGGT80250.053.835259
CGAACTT16650.052.71972768
GCCGGTT15950.051.8292583
CTACGGC12550.050.7773936
CGCGGTG86300.050.50030510
GGGGACT45300.050.428891
TGGCGCG86750.050.38677615
ACTCTTC48650.048.852945
GCCATAC13100.048.65357211
CCGGTTC17600.048.1445244
CTTAGGC17400.048.12460318
TTAGGCA17350.048.07396319
CGGCCAT13400.047.5643129
CATATTG18100.047.5157557
TCTGGTC49800.047.4579610
Produced by FastQC (version 0.11.5)